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  • Slides were viewed on a Leica DM 5000 microscope equipped with a Leica 350DX camera (Leica Microsystems, Inc), and the staining in each section was evaluated independently by several observers. Additional protein validation was limited by tissue availability.
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  • Cells were examined at room temperature using an imaging system (DM 5000; Leica Microsystems, Inc.) with a 100× HCX PL FLUOTAR objective (Leica Microsystems, Inc.). The images were captured with a digital camera (DFC 340 FX Alpha; Leica Microsystems, Inc.) and processed using AF6000 (Leica Microsystems, Inc.).
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